Dip-pen Nanolithography and Microcontact printing were used to fabricate mesopatterned substrates for cell differentiation experiments. A biotin-thiol was patterned on gold substrates and subsequently functionalised with streptavidin and biotinylated bone morphogenetic protein-2 (BMP-2). The feasibility of mesopatterned substrates containing immobilised BMP-2 was proven by obtaining similar differentiation outcomes compared to the growth factor in solution. Therefore, these substrates might be suitable for replacing conventional experiments with BMP-2 in solution.
Mesopattern of Immobilized Bone Morphogenetic Protein-2 Created by Microcontact Printing and Dip-pen Nanolithography Influence C2C12 Cell Fate
S. Oberhansl, A. G. Castaño, A. Lagunas, E. Prats-Alfonso, M. Hirtz, F. Albericio, H. Fuchs, J. Samitier, E. Martinez
RSC Adv. 4 (2014) 56809-56815