Multi-color patterning by polymer pen lithography (PPL) was used to fabricate covalently immobilized fluorophor- and oligonucleotide arrays with up to five different components. The oligonucleotide arrays offer a virtually unlimited inventory of orthogonal binding tags for self-assembly of proteins as demonstrated by use of the arrays to monitor cell-protein interactions of MCF7 cells.
Multi-Color Polymer Pen Lithography for Oligonucleotide Arrays
R. Kumar, S. Weigel, R. Meyer, C. M. Niemeyer, H. Fuchs, M. Hirtz
Chem. Commun. 52 (2016) 12310-12313